Chr-A Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9160-50, MB9160-100

Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10645(Human) P26339(Mouse) P10354(Rat)

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC

All Applications: WB:1:500
ICC:1:50-1:200
IHC:1:50-1:200

Background: Chromogranins (secretogranins) are acidic glycoproteins that localize within secretory granules of endocrine, neuroendocrine and neuronal tissue. Family members include chromogranin A (Chr-A), chromogranin B (Chr-B, also known as secretogranin I) chromogranin C (also known as secretogranin II or Sg II), secretogranin III (Sg III or SCG3). High levels of Chr-A expression is a characteristic of neuroendocrine tumors. Pancreastatin is a peptide derived from Chr-A which inhibits insulin secretion, exocrine pancreatic secretion and gastric acid secretion. Pancreastatin exists as two forms; the major form is expressed in stomach and colon extracts. In neuroendocrine cells the level Sg II has been shown to increase four-fold in response to histamine, while levels of Chr-A and Chr-B showed little or no increase. Sg III is an acidic secretory protein expressed in neuronal and endocrine cells. In the anterior lobe of the rat pituitary gland, Sg III is present in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, though not in somatotropes. Sg III and carboxypeptidase E (CPE) bind specifically to cholesterol-rich secretory granule (SG) membranes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Chr-A monoclonal antibody detects endogenous levels of Chr-A protein.

Bioworld Molecular Weight: 86 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Chromogranin A on PC-12 using anti-Chromogranin A antibody at 1/500 dilution., ICC staining Chromogranin A(red) in Hela cells. The nuclear counter stain is DAPI(blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CgA, SP-I, CHGA, beta Granin, betagranin (N-terminal fragment of chromogranin A), catestatin, CgA, CHG A, Chga, chromofungin, Chromogranin A, Chromogranin A parathyroid secretory protein 1, Chromogranin A precursor, ChromograninA, CMGA, ER-37, Pancreastatin, Parastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Secretory protein I, SP I, SP-I, SP1, SPI, Vasostatin, Vasostatin I, Vasostatin II,

Immunogen: Peptide

Conjugate: Unconjugated

Modification: Unmodified