CD47 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9137-50, MB9137-100

Product: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q08722(Human) Q61735(Mouse)

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, IHC FC

All Applications: WB:1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: CD47 is an integral membrane protein that plays a role in the regulation of cation fluxes across cell membranes. Specifically, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix. It is also a receptor for the C-terminal cell binding domain of thrombospondin (SIRP). CD47 is absent from Rh-null erythrocytes, but does play a role in cell adhesion in non-erythroid cells and may prevent premature elimination of erythrocytes. It may also be involved in membrane permeability changes following viral infection. CD47 is expressed on hemopoietic cells, epithelial cells, endothelial cells and fibroblasts and is strongly expressed in brain and mesenchymal cells.

Purification and Purity: ProG affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CD47 monoclonal antibody detects endogenous levels of CD47 protein.

Bioworld Molecular Weight: Predicted band 35 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CD47 on NIH-3T3 cell (1), Mouse brain (2), Hela cell (3) and K562 cell (4) lysates using anti-CD47 antibody at 1/500 dilution., Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD47 antibody. Counter stained with hematoxylin.

Alternative Name: IAP, CD47, MER6, Antigen identified by monoclonal antibody 1D8, Antigenic surface determinant protein OA3, CD 47, Cd47, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 antigen, CD47 glycoprotein, CD47 molecule, CD47, IAP, Integrin Associated Protein, Integrin associated signal transducer, Integrin-associated protein, Leukocyte surface antigen CD47, MER 6, MER6, OA 3, OA3, OTTHUMP00000041152, OTTHUMP00000041153, Protein MER6, Rh related antigen, Surface antigen identified by monoclonal antibody 1D8,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified