Ras Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9812M-50, BS9812M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01111/P01112/P01116

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebra fish

Applications: WB, IP, , FC

All Applications: WB: 1:1000-1:5000
IP: 1:50-1:200
FC: 1:10-1:100

Background: Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody is predicted to react with H-Ras, N-Ras and K-Ras.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Ras Rabbit mAb at 1:1000 dilution
Lane 1: MCF7 whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: The brain tissue lysate of Mouse, Flow cytometric analysis of Hela cells with Ras antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Alternative Name: GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; HRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; NRAS; HRAS1; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras; GTPase KRas; KRAS; KRAS2, RASK2

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified